Nutrition Active Complex for Muscle Protection
Optimizes the performance and accelerates the recovery
The amount of scientific evidence on the health benefits of sport and physical activity is overwhelming: contribution to the well-being and prevention of chronic diseases. However, any period of intense or prolonged muscle activity can cause a decline in performance as well as muscle weakness.
SEPPIC designed a nutrition active to protect the muscle, optimize its performance and accelerate recovery, thus helping to maintain a regular sporting activity.
Muscle weakness, what’s the point?
There are two kinds of muscle weakness:
- Muscle fatigue if the weakness is largely and quickly reversible.
- Exercise-induced muscle damage (EIMD) if the weakness is slowly or poorly reversible and associated with structural changes. Muscle damage causes an immediate weakness, which can take a number of days to recover. In addition, the damaged muscles develop soreness, swelling and stiffness 1-2 days after exercise (delayed-onset muscle soreness or DOMS).
Several biological mechanisms are associated with muscle fatigue, including muscle acidosis and Adenosine Triphosphate depletion. Muscle acidosis comes from lactic acid production which occurs during glycolysis and can interfere with the mechanisms of muscle contractions. This indicates that aerobic ATP generation is too low ans has to be supported by anaerobic ATP generation. This mechanism can be followed by biomarkers such as lactate production by muscle cells.
Skeletal muscle in contractions also release myokines (cytokines produced by muscles). Among these myokines, interleukin-6 (IL-6) is exponentially produced in response to muscle contractions. It seems to act as an hormone in order to regulate the supply in glucose during physical exercise and is released by muscles or liver to maintain the glycemic homeostasis.
Muscle damages, associated with structural changes, induce the release of proteins from myofibers. Creatine kinase is leaked by muscle cells when damage occurs and is used as a biomarker of muscle damage as it is almost exclusively produced by muscle cells.
Development of a proprietary in vitro test to study muscle protection
SEPPIC investigated the protective effect of several ingredients on muscular fatigue and damages in a model of physical exercise, by developing an in vitro model consisting in human skeletal muscle cells (HSMC), differentiated in myotubes, and then challenged with A23187 calcium ionophore, mimicking a physical exercise. Ingredients were added to culture medium of HSMC 24 hours before A23187 challenge. Fatigue and damages were monitored by quantifying release of lactate and IL-6 in cell supernatants, and intracellular activity of creatine kinase.
A23187 significantly increased the release of lactate, and IL-6 and decreased the intracellular activity of creatine kinase in HSMC.
Muscle performance & recovery - design of the active complex
Among the tested ingredients, an active complex of polyphenols from wine extract, vitamin E and zinc was selected because it allowed a significant protection of the muscle cells against the effects of A23187 on the three muscle weakness biomarkers, in a higher way than the ingredients tested alone.
The active complex demonstrated its protective effect against muscle fatigue and damages. Specially designed for dietary supplements, it contributes to the optimization of performance and recovery during physical exercise.
From a manufacturing perspective, the active complex benefits from a perfect homogeneity and stability as well as an easy introduction in the different supplements forms (tablets, hard and capsules, powders for drinks preparation...). The recommended daily dose can easily fit in a single tablet or capsule for good consumer compliance.