Ambre De Pooter, Laetitia Cattuzzato, Catherine Kern, Sandy Dumont
2017, Journal of the International Society of Antioxidants
Period of intense or prolonged muscle activity can reduce performance and cause muscle weakness. If the weakness is quickly reversible, it is named fatigue. If the weakness is slowly or poorly reversible and associated with structural changes, it is described as muscle damage and can lead to soreness. We investigated the effect of wine polyphenols, vitamin E and zinc on human skeletal muscle cells (HSMC) incubated with A23187, a calcium ionophore that mimicks cellular effects of exercise- induced muscle fatigue and damage. Fatigue- and damage-like phenotypes were monitored by quantifying the secretion of lactate and IL-6, and the intracellular activity of creatine kinase. As expected, administration of A23187 increased levels of lactate and IL-6 in the medium and decreased the intracellular activity of creatine kinase in HSMC. Administration of either wine polyphenols, vitamin E or zinc alone, caused a very limited protection of HSMC from A23187-induced effects.
However, the simultaneous administration of all three compounds caused a marked and significant protection. These results demonstrate that a combination of wine polyphenols, vitamin E and zinc protect muscle cells from injury normally associated with fatigue and damage, and suggest the possibility that this combination could be used to optimize exercise performance and recovery.
Seppic, Innovation Direction, Castres, France