MONTANIDE adjuvant for antibodies production

The alternative to Freund’s adjuvants

SEPPIC has combined science, expertise and service in order to develop vaccine adjuvants. These adjuvants, registered under the trademark of MONTANIDE have been optimized in order to improve efficacy and stability of vaccine formulations and to reduce side effects. They are widely registered in commercial vaccines for various target species (pigs, cattle, poultry, fishes ...).

For antibodies production, we have selected a powerful adjuvant, MONTANIDE ISA 50 V2, proved to be as reliable as Freund’s adjuvant with:

Good efficiency
Low side effects
Easy handling
Improved syringeability

MONTANIDE ISA 50 V2 is a mineral oil based adjuvant that has been developed for the manufacture of water-in-oil (W/O) emulsions. It comprises an injectable pharmaceutical mineral oil and an extremely refined emulsifier obtained from mannitol and purified oleic acid of vegetable origin.

New packaging: MONTANIDE ISA 50 V2 is commercially available by box of 10 ampoules (8ml per ampoule). For any order, thanks to contact us by mail: montanide@airliquide.com

New vaccine manufacturing process

MONTANIDE ISA 50 V2 is used at a ratio of 50/50 (volume). SEPPIC takes pride in its new and simple protocol of good emulsion manufacture: the T-connector emulsifying protocol.

Step 1- Prepare one part of MONTANIDE ISA 50 V2 in a vial. Then add one part of antigen aqueous solution.

Step 2- Pump both liquids using a sterile syringe with a needle. Remove the air and discard the needle.

Step 3- Connect the syringe to the T-connector* as shown. Do the same with an empty syringe.

Emulsifying:

20 cycles** at low speed (40 seconds)
80 cycles at high speed (80 seconds)

Step 4- Disconnect the T-connector and add a needle to the syringe. It is ready to inject.

*T-connector: Braun Discofix-3 (Ref: 4095111)
**a cycle: the passing of the formulation from one syringe to the other and back
If you need more details, do not hesitate to contact us: we will be pleased to provide them.

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Events

Jun 10, 2012
22nd International Pig Veterinary Society Congress
This year, this major annual event is held in Korea and it will gather the main global actors on pig health. SEPPIC will...
Aug 4, 2012
American Association of Avian Pathologists
The AAAP meeting is a major US congress focussing on poultry health. Seppic will present new results on adjuvants for av...

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